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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which ends up in chromosome instability, and thus may possibly add to tumorigenesis. This protein was discovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in advertising mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two supplemental splice variants were being claimed but specific nucleotide sequence details is not still obtainable.
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Blocking peptides are peptides that bind specifically to your concentrate on antibody and block antibody binding. These peptide ordinarily incorporates the epitope identified by the antibody.
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This manual outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This manual outlines more attainable optimization of mobile and immunoassay parameters to make certain the best possible outcomes are...
Antibodies sure to the blocking peptide no more bind on the epitope to the focus on protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.
Target Facts Cyclin E belongs on the really conserved cyclin household, whose customers are characterized by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which contribute to the temporal coordination of each and every mitotic occasion. Cyclin E forms a fancy with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of get more info receptor activation by both antibodies...
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